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KMID : 0371619870030010125
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Journal of Wonkwang Medical Science
1987 Volume.3 No. 1 p.125 ~ p.133
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A Morphological Study on the Effect of Hexavalent Chromium on the Early Development Stages of Mouse Embryos in vitro
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朴承澤/Park, Seung Taeck
金正雄/禹元洪/崔玟圭/鄭然泰/Kim, Jung Oong/Woo, Won Hong/Choi, Min Kyu/Chung, Yeun Tai
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Abstract
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Mouse two-cell embryos were cultured in the medium containing various concentrations of Cr" for 72 hours in order to elucidate the cytotoxicity of heavy metal ion and also biochemical observation was performed to measure the protein synthesis in early embryos which were injected with 3H?leucine for two hours.
The results were summerized as follows.
1. Chromium ion(Cr6´) inhibited remakably the development of mouse early embryos at 1 ppn, when two-cell embryos were cultured for 72 hours at various concentrations of chromium ion.
2. Chromium ion(Cr6´) retarded or suppressed the cleavage of early embryos. Fusion, fragmentation, cytolysis and developmental retardation of blastomeres were observed in the each developmental stage of embryonal cells.
3. Chromium ion(Cr6´) suppressed markedly the incorporation of H?leucine, but didn´t give an influence on the uptake of precursor by examing the two-cell embryos exposed to chromium ion for 6 and 24 hours respectively.
From this result, it suggest that chromium ion(Cr6´) might suppress the development of early embryos by inhibiting the protein synthusis.
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